Huang, A. (2021). The effect of the combined use of garlic extract and sodium salicylate on the inhibition and eradication of Staphylococcus epidermidis biofilms grown on titanium. The Young Researcher, 5(1), 148-163.
Implant associated infections (IAIs) place stress on patients and burden the healthcare system, and Staphylococcus epidermidis is a common pathogen involved. This study analyzed the effects of aqueous garlic extract (AGE) and sodium salicylate (SOD SAL) on the inhibition and eradication of S. epidermidis biofilms grown on titanium to find a potential remedy for IAIs. In the inhibition assay, bacterial suspensions were incubated with titanium discs and either AGE, SOD SAL, or both. In the eradication assay, bacteria cultured on titanium were treated with the treatments. The absorbance in optical density (OD) of each sample after treatment was analyzed using a spectrophotometer. AGE inhibited >40% of biofilm growth, whereas AGE and the combination treatment eradicated 17% of bacteria. It was concluded that AGE is better at inhibiting biofilm growth, but all treatments showed the same efficacy in eradicating pre-grown biofilm. Therefore, AGE could be used to treat and prevent IAIs.
Keywords: Staphylococcus epidermidis, titanium, garlic extract, sodium salicylate